Journal: FEBS Open Bio

Article Title: Characterization of ribosome heterogeneity during endothelial to hematopoietic transition

doi: 10.1002/2211-5463.70078

Figure Lengend Snippet: The expression pattern of ribosomal proteins during EHT at the proteome level. (A) The distribution and quantity of RPL, RPS and RAP proteins were analyzed using bulk‐cell proteomic data during EHT (EC: endothelial cell, n = 3; HEC: hemogenic endothelial cell, n = 3; HC: hematopoietic cell, n = 3). (B) The average expression level of RPL, RPS and RAP proteins during EHT. (C) The stage‐specific ribosome‐related proteins during EHT. The relative fold change of average protein expression level was represented between adjacent populations. (D, E) The differentially expressed pattern of RPS15a, RPL27 and RACK1 at transcriptome (D) and proteome (E) levels.

Article Snippet: The primary antibodies were as follows: RACK1 (Proteintech, Rosemont, IL, USA; dilution 1 : 200), RPL27 (Proteintech; dilution 1 : 50), RPS15a (Boster, Pleasanton, CA, USA; dilution 1 : 50) and RPS6 (Proteintech; dilution 1 : 50).

Techniques: Expressing

Journal: FEBS Open Bio

Article Title: Characterization of ribosome heterogeneity during endothelial to hematopoietic transition

doi: 10.1002/2211-5463.70078

Figure Lengend Snippet: Quantitative analysis of ribosomal protein expression by immunofluorescence. (A) Representative immunofluorescence images showing subcellular localization of RACK1, RPL27, RPS15a and RPS6 in AECs (CD41 − CD43 − CD45 − CD31 + CD201 − Kit − CD44 + ), HECs (CD41 − CD43 − CD45 − CD31 + CD201 + Kit + CD44 + ) and HCs (CD45 + Kit + ). Scale bars = 10 μm. (B) Quantitative analysis of ribosomal protein expression through immunofluorescence signal intensity measurement. Data were collected from single AEC ( n = 6), HEC ( n = 6) and HC ( n = 5). Data are presented as the mean ± SD and analyzed by unpaired two‐tailed Student's t ‐test. Data were collected from three independent experiments.

Article Snippet: The primary antibodies were as follows: RACK1 (Proteintech, Rosemont, IL, USA; dilution 1 : 200), RPL27 (Proteintech; dilution 1 : 50), RPS15a (Boster, Pleasanton, CA, USA; dilution 1 : 50) and RPS6 (Proteintech; dilution 1 : 50).

Techniques: Expressing, Immunofluorescence, Two Tailed Test

Journal: Neurological Research and Practice

Article Title: Anti-Homer-3 antibodies in cerebrospinal fluid and serum samples from a 58-year-old woman with subacute cerebellar degeneration and diffuse breast adenocarcinoma

doi: 10.1186/s42466-022-00194-9

Figure Lengend Snippet: Immunofluorescence analysis of the patient’s samples (rows 1 and 2), as well as two additional samples from two other patients, known to be positive (row 3) or negative (row 4) for anti-Homer-3 antibodies. Numbers in figure panels indicate respective dilution of samples. HEK293 controls were included for all incubated samples (right column) and did not show fluorescence

Article Snippet: Indirect immunofluorescence test (IIFT) on HEK293 cells ectopically expressing selected antigens (EUROIMMUN Medizinische Labordiagnostika AG, Lübeck, Germany) revealed the presence of anti-Homer-3 antibodies (IgG) with a titer of 1:32,000 in serum and 1:100 in CSF.

Techniques: Immunofluorescence, Incubation, Fluorescence